Device
Part:BBa_I715054
Designed by: Andrew Martens Group: iGEM07_Davidson_Missouri_W (2007-11-07)
Test Trp anti-terminator/terminator
This part is designed to test the functionality of the trp operon regulatory sequence which uses transcriptional attenuation (via the "anti-termination" mechanism) to regulate gene expression.
Usage and Biology
In the presence of tryptophan, the trp regulatory sequence will attenuate transcription, and GFP will not be transcribed. This construct serves as a means of testing the functionality of the trp regulatory sequence by comparing GFP expression with different environmental concentrations of tryptophan amino acid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1012
Functional Parameters
[edit]
Categories
Parameters
None |